Dynactin suppresses the retrograde movement of apically localized mRNA in Drosophila blastoderm embryos

Dynactin suppresses the retrograde movement of apically localized mRNA in Drosophila blastoderm embryos
By Georgia Vendra¹, Russell S. Hamilton^1,2 & Ilan Davis^1,2,3
¹Wellcome Trust Centre for Cell Biology, Michael Swann building, University of Edinburgh, Edinburgh EH9 3JR, UK
²Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
³Correspondence and requests for materials should be addressed to: Ilan.Davis -at- bioc.ox.ac.uk

Lab Web Page: www.ilandavis.com

Main Article Link: RNA Journal via [DOI]

Supplementary Materials: http://www.ParticleStats.com/Vendra2007RNA

Supplementary Figures and Tables:

SupplementaryFigure_1.pdf
SupplementaryFigure_2.pdf
SupplementaryFigure_3.pdf
SupplementaryTable_1.pdf

Supplementary Movies:
SupplementaryMovieLegends.pdf

Supplementary Movie 1. ftz RNA particle movements in wild type embryos. In vitro transcribed, fluorescently labeled ftz RNA moves bi-directionally in wild type syncytial blastoderm embryos. The apical side of the embryo is up and the basal is down. A relatively smooth minus run is marked in red and another minus run, interrupted by pauses and shorter or longer backward runs is marked in green. Movie is speeded up approximately 2.8 times and represents 80sec. Vendra2007_movie1.mov

Supplementary Movie 2. ftz RNA particle trails in wild type embryos Trail movie of a selected region of movie 1. Most of the ftz RNA particles shown move towards the minus ends of MTs. Two particles display plus runs. Movie is speeded up approximately 2.8 times. Vendra2007_movie2.avi

Supplementary Movie 3. ftz RNA movement in Khc mutants In vitro transcribed, fluorescently labeled ftz RNA moves bi-directionally in Khc27 syncytial blastoderm embryos. The apical side of the embryo is up and the basal is down. Movie is speeded up approximately 6.5 times and represents 41sec. Vendra2007_movie3.mov

Supplementary Movie 4. ftz RNA movement in KLP64D mutants In vitro transcribed, fluorescently labeled ftz RNA moves bi-directionally in KLP64D^k1 syncytial blastoderm embryos. The apical side of the embryo is up and the basal is down. Movie is speeded up approximately 6 times and represents 77sec. Vendra2007_movie4.mov

Supplementary Movie 5. ftz RNA transport in the presence of the kinesin inhibitor AMP-PNP. In vitro transcribed, fluorescently labeled ftz RNA moves bi-directionally in syncytial blastoderm embryos preinjected with AMP-PNP. The apical side of the embryo is up and the basal is down. Movie represents 142sec and is speeded up approximately 9 times. Vendra2007_movie5.mov

Supplementary Movie 6. ftz RNA transport in Glued mutants In vitro transcribed, fluorescently labeled ftz RNA moves more frequently towards the plus end of MTs in Glued1 syncytial blastoderm embryos. The apical side of the embryo is up and the basal is down. Movie is speeded up approximately 5 times and represents 63sec. Vendra2007_movie6.mov

Supplementary Data & Software:

The ParticleStats software and Excel datafiles are available from www.ParticleStats.com

Supplementary Movies:	SupplementaryMovieLegends.pdf
*Supplementary Movie 1. ftz* RNA particle movements in wild type embryos.** In vitro transcribed, fluorescently labeled ftz RNA moves bi-directionally in wild type syncytial blastoderm embryos. The apical side of the embryo is up and the basal is down. A relatively smooth minus run is marked in red and another minus run, interrupted by pauses and shorter or longer backward runs is marked in green. Movie is speeded up approximately 2.8 times and represents 80sec.	Vendra2007_movie1.mov
Supplementary Movie 2. ftz RNA particle trails in wild type embryos Trail movie of a selected region of movie 1. Most of the ftz RNA particles shown move towards the minus ends of MTs. Two particles display plus runs. Movie is speeded up approximately 2.8 times.	Vendra2007_movie2.avi
Supplementary Movie 3. ftz RNA movement in Khc mutants In vitro transcribed, fluorescently labeled ftz RNA moves bi-directionally in Khc27 syncytial blastoderm embryos. The apical side of the embryo is up and the basal is down. Movie is speeded up approximately 6.5 times and represents 41sec.	Vendra2007_movie3.mov
Supplementary Movie 4. ftz RNA movement in KLP64D mutants In vitro transcribed, fluorescently labeled ftz RNA moves bi-directionally in KLP64D^k1 syncytial blastoderm embryos. The apical side of the embryo is up and the basal is down. Movie is speeded up approximately 6 times and represents 77sec.	Vendra2007_movie4.mov
Supplementary Movie 5. ftz RNA transport in the presence of the kinesin inhibitor AMP-PNP. In vitro transcribed, fluorescently labeled ftz RNA moves bi-directionally in syncytial blastoderm embryos preinjected with AMP-PNP. The apical side of the embryo is up and the basal is down. Movie represents 142sec and is speeded up approximately 9 times.	Vendra2007_movie5.mov
Supplementary Movie 6. ftz RNA transport in Glued mutants In vitro transcribed, fluorescently labeled ftz RNA moves more frequently towards the plus end of MTs in Glued1 syncytial blastoderm embryos. The apical side of the embryo is up and the basal is down. Movie is speeded up approximately 5 times and represents 63sec.	Vendra2007_movie6.mov